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KMID : 0615720150320030027
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Jouranl of Korean Acupuncture and Moxibustion society
2015 Volume.32 No. 3 p.27 ~ p.40
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Inhibitory Effects of Yongbu-tang on Osteoclast Differentiation and Bone Resorption
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Lee Jeong-Ju
Jo So-Hyun
Park Min-Cheol
Cho Eun-Hee
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Abstract
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Objectives: This study was performed to evaluate the effects of water extract of Cervi Parvum Cornu(CPC), Aconiti Lateralis Radix Preparata(ALR), and Yongbu-tang(YBT) on suppression of the receptor activator of nuclear factor kappa-B ligand(RANKL)-induced osteoclast differentiation and bone resorption.
Methods: The effects of CPC, ALR, YBT extracts on osteoclast differentiation were determined by culture of bone marrow macrophage(BMM). The mRNA expression levels of the nuclear factor of activated T-cells cytoplasmic 1(NFATc1), c-Fos and tartrate-resistant acid phosphatase(TRAP) in BMMs were analyzed by reverse transcriptase polymerase chain reaction(RT-PCR). Similarly, the protein expression levels of NFATc1, c-Fos, mitogen-activated protein kinase(MAPK)s and β-actin in cell lysates were measured by western blotting. In addition, effects of CPC, ALR and YBT extracts were determined by means of Lipopolysaccharide(LPS)-induced bone-loss with mice.
Results:CPC, ALR and YBT extracts showed remarkable inhibition on RANKL-induced osteoclast differentiation without cytotoxicity. CPC and ALR extracts significantly reduced the protein expression level of NFATc1. YBT extract significantly reduced the mRNA expression levels of c-Fos, NFATc1 and the protein expression levels of c-Fos, NFATc1, AKT, p38, c-Jun N-terminal kinase(JNK). Further, YBT extract suppressed degradation of I-κB. And ALR extract significantly restored the bone erosion by LPS treatment in mice.
Conclusions: YBT extract showed more remarkable inhibition on osteoclast differentiation than CPC and ALR extracts in vitro. ALR extract showed remarkable inhibition on bone resorption in vivo. Thus, YBT extract can be a useful treatment for bone-loss diseases such as osteo porosis.
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KEYWORD
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Cervi Parvum Cornu, Aconiti Lateralis Radix Preparata, Yongbu-tang, Osteoporosis, Osteoclast Differentiation, Bone resorption
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